Plastic Contaminant Masquerades as DNA in Mutation Detection by Denaturing HPLC
نویسندگان
چکیده
منابع مشابه
Plastic contaminant masquerades as DNA in mutation detection by denaturing HPLC.
Denaturing high-performance liquid chromatography (DHPLC) has quickly become popular as a tool for the automated discovery of single base substitutions as well as small insertions and deletions (1,2). Under partially denaturing conditions, heteroduplices are retained for a shorter time than their corresponding homoduplices on a DNA separation matrix. We have identified a contaminant emanating f...
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BACKGROUND In recent years, denaturing HPLC (DHPLC) has been widely used to screen the whole mitochondrial genome or specific regions of the genome for DNA mutations. The quantification and mathematical modeling of DHPLC results is, however, underexplored. METHODS We generated site-directed mutants containing some common mutations in the mitochondrial DNA (mtDNA) tRNA(leu) region with differe...
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hydrochlorothiazide (hct) is a diuretic agent which is shown to be effective in the treatment of hypertension. literature reports have demonstrated that urinary excretion data may be used to assess the bioavailability of various formulations containing this thiazide. also hct consumption by the athletes is one of the drugs which should be regulated by world anti-doping agency (wada), because of...
Emergence of Ofloxacin Resistance in Mycobacterium tuberculosis Clinical Isolates from China: gyrA Mutation Analysis by Denaturing HPLC and DNA Sequencing
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متن کاملHigh-resolution SNP mapping by denaturing HPLC.
With the availability of complete genome sequences, new rapid and reliable strategies for positional cloning become possible. Single-nucleotide polymorphisms (SNPs) permit the mapping of mutations at a resolution not amenable to classical genetics. Here we describe a SNP mapping procedure that relies on resolving polymorphisms by denaturing HPLC without the necessity of determining the nature o...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2003
ISSN: 0736-6205,1940-9818
DOI: 10.2144/03341bm09